Engineering and expression of sequence-specific DNA-binding zinc finger protein.
- Author:
Yong WEI
1
;
Dajun YING
;
Chunli HOU
;
Chuhong ZHU
;
Xiaoping CUI
;
Yan XING
;
Hongfeng GUO
Author Information
1. Key Laboratory of Biomechanics and Tissue Engineering, Chongqing, China.
- Publication Type:Journal Article
- MeSH:
Amino Acid Sequence;
Base Sequence;
DNA-Binding Proteins;
chemistry;
genetics;
Humans;
Molecular Sequence Data;
Protein Binding;
Protein Engineering;
methods;
Transcription Factors;
chemistry;
genetics;
Zinc Fingers;
genetics
- From:
Journal of Biomedical Engineering
2008;25(3):662-667
- CountryChina
- Language:Chinese
-
Abstract:
This experiment was aimed to create A20 gene site-specific zinc finger DNA-binding protein. The sequence of A20 gene promoter was analyzed with bioinformatics means and submitted to ZF Tools Server at TSRI. Using the database of the web site, we determined the A20 gene valid target sites and designed the amino acid sequence of zinc finger protein predicted to be bound to the target site. And then, the structure of the protein sequence was analyzed and homology was modeled with various bioinformatics means. Based on the characteristic of this protein, the prokaryotic expression vector pTYB11-ZFP was constructed and expressed. Thus, the artificial zinc finger protein that recognized A20 specific sequence was designed, and expressed in Escherichia coli. The results indicate that it is feasible to design engineered artificial Zinc finger proteins by means of bioinformatics.
