Clone of human xeroderma pigmentosum group D cDNA and analysis of its expression and function.
- Author:
Lei TANG
1
;
Jixiang ZHANG
;
Ying XIONG
Author Information
1. The Second Affiliated Hospital of Jiangxi Medicine College, The Key Laboratory of Molecular Medicine of Jiangxi Province, Nanchang 330006, China.
- Publication Type:Journal Article
- MeSH:
Cloning, Molecular;
DNA Repair;
Female;
HeLa Cells;
Humans;
Liver Neoplasms;
genetics;
Recombinant Proteins;
genetics;
metabolism;
Trans-Activators;
genetics;
Transcription Factor TFIIH;
genetics;
Transcription, Genetic;
Transfection;
Viral Regulatory and Accessory Proteins;
genetics;
Xeroderma Pigmentosum Group D Protein;
genetics;
metabolism
- From:
Journal of Biomedical Engineering
2008;25(3):668-672
- CountryChina
- Language:Chinese
-
Abstract:
Xeroderma pigmentosum group D (XPD) gene is the second subunit of basic transcript factor TFII H; it plays an important role in transcription and nucleotide excision repair. In this study, using the total RNA extracted from HeLa cells, we cloned the human full length XPD by RT-PCR and inserted it into the pEGFP-N2 plasmid vector which expressed the green fluorescence protein (GFP). Then the recombinant plasmid pEGFP-N2/XPD was transfected into the human hepatoma carcinoma cell Hep3B integrated with HBx protein,and we analysed the expression of HBx and the proliferative ability of recombinant cells. The data collected from this study could serve as a physical basis on which to further investigate the biological activities of XPD.
