Freeze-drying of oxygen-carrying substances and storage stability of the freeze-dried solids.
- Author:
Zhi WANG
1
;
Fengjuan LI
;
Honghui ZHANG
;
Rong YU
;
Chengmin YANG
Author Information
1. West China School of Pharmacy, Sichuan University, Chengdu 610041, China.
- Publication Type:Journal Article
- MeSH:
Chromatography, High Pressure Liquid;
Drug Stability;
Drug Storage;
Electrophoresis, Polyacrylamide Gel;
Female;
Freeze Drying;
Hemerythrin;
analysis;
Hemoglobins;
chemistry;
Humans;
Methemoglobin;
analysis;
Placenta;
chemistry;
Sucrose;
pharmacology
- From:
Journal of Biomedical Engineering
2008;25(3):678-693
- CountryChina
- Language:Chinese
-
Abstract:
Oxygen-carrying substances based on poly-placental hemoglobin were put into freeze-drying. Sucrose was chosen to inhibit the methmoglobin(MetHb) formation. MetHb content, ultravioletes(UV) spectrum, Sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE), High performance liquid chromatography (HPLC) were monitored during freeze-drying. As a result, when the mass ratio of sucrose to protein was above 0.5, MetHb formation was under control;the characteristic absorption of UV spectrum, SDS-PAGE and HPLC showed no visible change. Freeze-dried solids were kept under room temperature and refrigerator for 3 months. As a result, MetHb formation depended upon storage temperature and the mass ratio of sucrose to protein. For group C in which the mass ratio of sucrose to protein was 1.0; there is no marked change in MetHb content, UV spectrum, SDS-PAGE and HPLC after 3 months.
