Effects of serum containing oral liquid of Guilu-Erxian on the therapy of osteoporosis at the cellular level.
- Author:
Cuiping MAO
1
;
Yun ZHANG
;
Weimin YAN
;
Xiaoxiang ZHENG
Author Information
1. Key Laboratory of Biomedical Engineering of Ministry of Education, Zhejiang University, Hangzhou 310027, China.
- Publication Type:Journal Article
- MeSH:
Acid Phosphatase;
metabolism;
Alkaline Phosphatase;
metabolism;
Animals;
Animals, Newborn;
Cell Proliferation;
Cells, Cultured;
Drugs, Chinese Herbal;
pharmacology;
therapeutic use;
Insulin-Like Growth Factor I;
metabolism;
Male;
Osteoblasts;
cytology;
metabolism;
Osteoclasts;
cytology;
metabolism;
Osteoporosis;
drug therapy;
Phytotherapy;
Rats;
Rats, Sprague-Dawley;
Serum
- From:
Journal of Biomedical Engineering
2008;25(4):897-902
- CountryChina
- Language:Chinese
-
Abstract:
This study sought to assess the effects of serum containing Guilu-Erxian (GLEX) on treating osteoporosis at the cellular level. First, Enzyme-digestion was used to obtain osteoblasts from newborn SD rats. Alkaline phosphatase staining was used to identify osteoblasts. The proliferation and the secretion of Insulin Like Growth Factor-1 (IGF-1) of osteoblasts were examined. Second, osteoclasts were generated by inducing bone marrow cells of SD male rat (6-9 weeks) with 1,25(OH)2D3. The osteoclasts were identified through tartrate-resistant acid phosphatase (TRAP) staining. Bone pits of osteoclasts were observed through Electron Scanning Microscope. At the same time, TRAP(+) cells with more than two nuclei and TRAP activity of osteoclasts were examined. Last, serum containing Guilu-Erxian was made through serum pharmacology. And we observed the effects of serum containing Guilu-Erxian on the function of osteoblasts and osteoclasts. We found that high doses of serum containing Guilu-Erxian had obvious effects on stimulating the multiplication and capability of secreting IGF-1 of osteoblasts cultured in vitro. Middle doses obviously inhibited the formation of osteoclasts and bone pits; TRAP activity of osteoclasts was also lowered clearly. This study indicated that Guilu-Erxian has positive effects on treating osteoporosis.
