Influence of curcumin--loaded poly (lactide-co-glycolide) films on the proliferation of vascular smooth muscle cells.
- Author:
Ling REN
1
;
Jin WANG
;
Jiaju TANG
;
Changjiang PAN
;
Nan HUANG
Author Information
1. Key Lab for Advanced Technologies of Materials of Ministry of education, Southwest Jiatong University, Chengdu, China.
- Publication Type:Journal Article
- MeSH:
Angioplasty, Balloon, Coronary;
Animals;
Carotid Arteries;
cytology;
Cell Proliferation;
drug effects;
Cells, Cultured;
Coated Materials, Biocompatible;
pharmacology;
Coronary Restenosis;
prevention & control;
Curcumin;
pharmacology;
Drug-Eluting Stents;
Lactic Acid;
pharmacology;
Muscle, Smooth, Vascular;
cytology;
Polyglycolic Acid;
pharmacology;
Rats
- From:
Journal of Biomedical Engineering
2008;25(4):874-878
- CountryChina
- Language:Chinese
-
Abstract:
In-stent restenosis is the major problem of percutaneous coronary interventions. Drug-eluting stent became a landmark in the treatment of coronary disease. Curcumin could be used for drug-eluting stent due to its antithrombogenity and antiproliferative properties. In this paper, 3-(4, 5-Dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT) assays were performed to decide the optimal concentration of curcumin for inhibiting the proliferation of vascular smooth muscle cells (VSMC). The result disclosed that more than 80% of VSMC were inhibited when the concentration of curcumin ranged from 2.5 microg/ml to 10 microg/ml (P < 0.05, compared to ethanol). Three weight percent curcumin-loaded films (3wt%, 5wt%, 8wt%) were prepared using a biodegradable polymer (poly (lactic acid-co-glycol acid), PLGA) as the carrier of curcumin. The release of lactate dehydrogenase (LDH) was used to evaluate the immediate toxicity of the curcumin-loaded PLGA films, and the three concentration curcumin-loaded films were revealed to be of no acute toxicity to the smooth muscle cells. The results of Alamar Blue test indicated that the curcumin-loaded films had better antiproliferation effect than did the 316 stainless steel (SS). Therefore, these films may be used for stent coating to inhibit the in-stent restenosis induced by VSMC proliferation.
