Synthesis and application of the polyacrylamide beads acting as LDL adsorbent's matrices.
- Author:
Xixun YU
1
;
Li LI
;
Yilun YUE
;
Huaiqing CHEN
Author Information
1. Institute of Biomedical Engineering, West China Center of Medical Sciences, Sichuan University, Chengdu 610041, China.
- Publication Type:Journal Article
- MeSH:
Acrylic Resins;
chemical synthesis;
Adsorption;
Blood Component Removal;
methods;
Cross-Linking Reagents;
Hemoperfusion;
instrumentation;
methods;
Humans;
Lipoproteins, LDL;
Microspheres
- From:
Journal of Biomedical Engineering
2004;21(4):582-586
- CountryChina
- Language:Chinese
-
Abstract:
This study in pursuit of the synthetic technologies and structure characterization of polyacrylamide-based matrices (PAM beads) for low density lipoprotein (LDL) adsorbent and their adsorbability for LDL was intended for an experimental evidence of developing advanced matrices for LDL adsorbent. PAM beads were synthesized by inverse suspension polymerization, and their structure characterization was characterized by SEM, image analyzer and small angle X-ray scattering. The tripeptide serine-aspartic-glutamic acid (SDE) was coupled on the PAM beads to prepare the LDL adsorbents whose adsorbability for LDL was determined in vitro. The results showed that the PAM beads with the average size diameter 142.1 microm and the average pore diameter 119.8 nm could act as the matrices in accordance with the requirement of adsorbent for LDL. When the amount of acrylamide and the crosslinking agent N,N'-methylene-bis(acrylamide) was fixed, the average pore diameter decreased with the increase of the crosslinking agent content. Although the nonspecific binding of PAM beads for LDL was low, they could selectively adsorb LDL after coupling the SDE on the PAM beads.
