Quantification of the curing effects of phenanthridine on yeast prion PSI+.
- Author:
Zhengwei ZHONG
1
;
Lijie WANG
;
Hui XIE
;
Hui LI
;
Jianwei HE
;
Youtao SONG
Author Information
1. School of Life Science, Liaoning University, Shenyang 110036, Liaoning, China.
- Publication Type:Journal Article
- MeSH:
Computer Simulation;
Models, Biological;
Peptide Termination Factors;
metabolism;
Phenanthridines;
pharmacology;
Prions;
drug effects;
genetics;
metabolism;
Saccharomyces cerevisiae;
cytology;
drug effects;
metabolism;
Saccharomyces cerevisiae Proteins;
metabolism
- From:
Chinese Journal of Biotechnology
2012;28(6):737-746
- CountryChina
- Language:Chinese
-
Abstract:
In order to quantify the curing effects of phenanthridine on yeast prion, we introduced semi-denaturing agarose gel electrophoresis and fluorescence recovery after photobleaching techniques to quantify the curing effects of phenanthridine on yeast prion at the protein and cellular levels with the [PSI+] yeast strain expressing GFP-Sup35p (NGMC). The results showed that these two approaches could precisely quantify the curing effects of phenanthridine on [PSI+] cells. After a treatment for 1 through 5 days with phenanthridine, the curing rates of [PSI+] cells were 0%, 0%, 51.7%, 87.5% and 94.4%, respectively. Meanwhile, we quantified the sizes of Sup35p polymers in phenanthridine induced pink phenotype cells. The aggregation status in 1-2 days phenanthridine treated cells were similar to those in [PSI+] cells, while the aggregation status in 3-5 days phenanthridine treated cells were similar to those in [psi(-)] cells.
