Design and application of high-throughput screening tools: a review.
- Author:
Shuangyan TANG
1
;
Chaoning LIANG
;
Peixia JIANG
Author Information
1. Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China. tangsy@im.ac.cn
- Publication Type:Journal Article
- MeSH:
Directed Molecular Evolution;
methods;
High-Throughput Screening Assays;
methods;
Mutagenesis, Site-Directed;
methods;
Mutant Proteins;
genetics;
Protein Engineering;
methods
- From:
Chinese Journal of Biotechnology
2012;28(7):781-788
- CountryChina
- Language:Chinese
-
Abstract:
As an efficient and promising protein engineering strategy, directed evolution includes the construction of mutant libraries and screening of desirable mutants. A rapid and high-throughput screening method has played a critical role in the successful application of directed evolution strategy. We reviewed several high-throughput screening tools which have great potential to be applied in directed evolution. The development of powerful high-throughput screening tools will make great contributions to the advancement of protein engineering.
