Cloning and characterization of a thermostable urate oxidase from Microbacterium sp. strain ZZJ4-1.
- Author:
Pengcheng ZHANG
1
;
Xiangfeng LU
;
Qianyan LI
;
Xiaoqing LIN
;
Hui LIU
;
Xiaohang MA
Author Information
1. College of Life Sciences, Zhejiang University, Hangzhou 310058, Zhejiang, China.
- Publication Type:Journal Article
- MeSH:
Actinomycetales;
enzymology;
genetics;
Amino Acid Sequence;
Cloning, Molecular;
Enzyme Stability;
Escherichia coli;
genetics;
metabolism;
Genetic Vectors;
genetics;
Molecular Sequence Data;
Recombinant Proteins;
biosynthesis;
genetics;
Urate Oxidase;
genetics;
metabolism
- From:
Chinese Journal of Biotechnology
2012;28(7):813-822
- CountryChina
- Language:Chinese
-
Abstract:
In order to characterize a thermostable urate oxidase (Uox) from Microbacterium sp. strain ZZJ4-1, we cloned its gene (uox). The open reading frame of uox contained 894 base pairs and encoded a protein with 297 amino acids. Alignment of gene sequences indicated there was no obvious identity with the most reported uox and that 72% identity was found with uox from Arthrobacter globiformis. We inserted the gene into the plasmid pET-15b to construct an expression vector pET-15b-uox and got it induced expression in Escherichia coli BL21 (DE3). After the purification of the recombinant Uox by the HisBind column, we studied some properties of it. It was composed of subunits with a molecular mass of about 35 kDa. The optimal temperature and pH was 30 degrees C and pH 7.5. It was stable below 65 degrees C and from pH 8.5 to 11.0. The Km value was 0.22 mmol/L with the uric acid as the substrate. Ag+, Zn2+, CU2+ and SDS could totally inhibit its activity while Tween 20, Tween 80 and Triton X-100 had a slight promotion effect. The thermal stability of this enzyme was the most excellent among the reported recombinant Uox. Based on this property, it would be very useful in the application.
