Development and application of Saccharomyces cerevisiae cell-surface display for bioethanol production.
- Author:
Fei YANG
1
;
Meng CAO
;
Yi JIN
;
Xiushan YANG
;
Shen TIAN
Author Information
1. College of Life Science, Capital Normal University, Beijing 100048, China.
- Publication Type:Journal Article
- MeSH:
Biofuels;
analysis;
microbiology;
Ethanol;
isolation & purification;
metabolism;
Fermentation;
Metabolic Engineering;
methods;
Recombinant Proteins;
biosynthesis;
genetics;
Saccharomyces cerevisiae;
genetics;
metabolism
- From:
Chinese Journal of Biotechnology
2012;28(8):901-911
- CountryChina
- Language:Chinese
-
Abstract:
Saccharomyces cerevisiae is useful as a host for genetic engineering, since it allows the folding and glycosylation of expressed heterologous eukaryotic proteins and can be subjected to many genetic manipulations. Recent advancements in the yeast cell surface engineering developed strategies to genetically immobilize amylolytic, cellulolytic and xylanolytic enzymes on yeast cell surface for the production of fuel ethanol from biomass. We reviewed the basic principle and progress of S. cerevisiae cell-surface engineering and gave an insight into the recent technological developments in the production of bioethanol using surface engineered yeast.
