Over-expressed MiR-103 promotes porcine adipocyte differentiation.
- Author:
Meihang LI
1
;
Yang QIU
;
Shuai LIU
;
Peiyue DONG
;
Xiaomin NING
;
Yanjie LI
;
Gongshe YANG
;
Shiduo SUN
Author Information
1. Laboratory of Animal Fat Deposition and Muscle Development, Northwest A & F University, Yangling 712100, Shaanxi, China.
- Publication Type:Journal Article
- MeSH:
Adenoviridae;
genetics;
metabolism;
Adipocytes;
cytology;
metabolism;
Adipogenesis;
genetics;
Animals;
Base Sequence;
Cell Differentiation;
MicroRNAs;
genetics;
metabolism;
Molecular Sequence Data;
PPAR gamma;
genetics;
metabolism;
Primary Cell Culture;
RNA, Messenger;
genetics;
metabolism;
Swine;
Transfection
- From:
Chinese Journal of Biotechnology
2012;28(8):927-936
- CountryChina
- Language:Chinese
-
Abstract:
To clarify the function of miR-103 in the differentiation of porcine preadipocyte, we carried out real-time PCR to detect the expression pattern of miR-103 during adipogenesis, and clarified its expression tendency through cell differentiation. Then we used adenovirus that overexpressed miR-103 to infect porcine preadipocyte. Subsequently, mRNA and protein expression of adipogenesis marker--PPARgamma and aP2 was analyzed by real-time PCR and Western blotting. At last, Oil-Red O staining was used to detect lipids accumulation in the 8th day after adipogenic inducement. The expression of miR-103 increased during adipocyte differentiation; compared with the control, the preadipocyte infected by pAd-miR-103 had an elevated expression level of adipocyte marker gene PPARgamma, aP2, and obvious lipid droplet was seen in the 8th day after adipogenic inducement. These results showed that miR-103 can enhance adipogenesis in primary cultured porcine adipocytes.
