Enhanced cellulase production of Penicillium decumbens by knocking out CreB encoding a deubiquitination enzyme.
- Author:
Guangqi ZHOU
1
;
Jing LÜ
;
Zhonghai LI
;
Jingjing LI
;
Mingyu WANG
;
Yinbo QU
;
Lin XIAO
;
Shulin QIN
;
Haitao ZHAO
;
Ruirui XIA
;
Xu FANG
Author Information
1. School of Bioengineering, Dalian Polytechnic University, Dalian 116034, Liaoning, China.
- Publication Type:Journal Article
- MeSH:
Cellulase;
biosynthesis;
Endopeptidases;
genetics;
metabolism;
Gene Knockout Techniques;
Lignin;
metabolism;
Mutant Proteins;
metabolism;
Penicillium;
enzymology;
genetics;
Recombination, Genetic;
Ubiquitinated Proteins;
genetics;
Ubiquitination
- From:
Chinese Journal of Biotechnology
2012;28(8):959-972
- CountryChina
- Language:Chinese
-
Abstract:
Penicillium decumbens T. is an important filamentous fungus for the production of cellulases to effectively degrade lignocellulose for second generation biofuel production. In order to enhance the capability of Penicillium decumbens to produce cellulases, we constructed a creB (a deubiquitinating enzyme encoding gene) deletion cassette, and generated a creB knockout strain with homologous double crossover recombination. This mutation resulted in a detectable decrease of carbon catabolite repression (CCR) effect. The filter paper activity, endoglucanase activity, xylanase activity and exoglucanase activity of the deltacreB strain increased by 1.8, 1.71, 2.06 and 2.04 fold, respectively, when comparing with the parent strain Ku-39. A 2.68 fold increase of extracellular protein concentration was also observed. These results suggest that the deletion of creB results in CCR derepression. These data also suggest that CREB influences cellulase production of Penicillium decumbens. In generation, this study provides information that can be helpful for constructing cellulase hyper-producing strain.
