Screening of Clostridium strains through ribosome engineering for improved butanol production.
- Author:
Lijie CHEN
1
;
Guanglai SHANG
;
Wenjie YUAN
;
Youduo WU
;
Fengwu BAI
Author Information
1. School of Life Science and Biotechnology, Dalian University of Technology, Dalian 116024, Liaoning, China. ljchen@dlut.edu.cn
- Publication Type:Journal Article
- MeSH:
Butanols;
metabolism;
Clostridium acetobutylicum;
drug effects;
genetics;
metabolism;
Fermentation;
Genetic Engineering;
Mutation;
Recombinant Proteins;
biosynthesis;
genetics;
Ribosomes;
genetics;
Streptomycin;
pharmacology
- From:
Chinese Journal of Biotechnology
2012;28(9):1048-1058
- CountryChina
- Language:Chinese
-
Abstract:
We used ribosome engineering technology, with which antibiotic-resistant strains are resulted from mutations on microbial ribosome, to screen a high butanol-producing Clostridium strain. A novel mutant strain S3 with high butanol production and tolerance was obtained from the original Clostridium acetobutylicum L7 with the presence of mutagen of streptomycin. Butanol of 12.48 g/L and ethanol of 1.70 g/L were achieved in S3, 11.2% and 50%, respectively higher than the parent strain. The conversion rate of glucose to butanol increased from 0.19 to 0.22, and fermentation time was 9 h shorter. This caused an increase in butanol productivity by 30.5%, reaching 0.24 g/(Lh). The mutant butanol tolerance was increased from 12 g/L to 14 g/L, the viscosity of fermentation broth was dramatically decreased to 4 mPa/s, 60% lower than the parent strain. In addition, the genetic stability of mutant strain S3 was also favorable. These results demonstrate that ribosome engineering technology may be a promising process for developing high butanol-producing strains.
