Heterologous expression of stearoyl-CoA desaturase-1 in Lactococcus lactis NZ3900.
- Author:
Lamei WANG
1
;
Shili LI
;
Kemian GOU
;
Yuzhu LUO
Author Information
1. College Animal Science and Technology, Gansu Agricultural University, Lanzhou 730070, Gansu, China.
- Publication Type:Journal Article
- MeSH:
Electroporation;
Humans;
Lactococcus lactis;
genetics;
metabolism;
Mutagenesis, Insertional;
Nisin;
pharmacology;
Recombinant Proteins;
biosynthesis;
genetics;
Stearoyl-CoA Desaturase;
biosynthesis;
genetics
- From:
Chinese Journal of Biotechnology
2012;28(9):1106-1117
- CountryChina
- Language:Chinese
-
Abstract:
The possibility of heterologous expression of human Stearoyl-CoA Desaturase (scd1) was investigated. The scd1 encoding sequence was inserted into the pNZ8149 to generate the pNZ8149-scd1 expression plasmids. Then we introduced the pNZ8149-scd1 construct into the Lactococcus lactis NZ3900 to investigate its enzyme activity. The results show that heterologous expressed SCD1 enzyme resulted in a 92%-169% increase in the C16:1n-7 and a 53-127% increase in the C18:1n-7 (P<0.05). The SCD1 enzyme was capable of producing n-7 fatty acids in Lactococcus lactis efficiently. It also suggests that the fatty acid desaturases can be heterologous expressed in Lactococcus lactis to produce the helpful fatty acids.
