Heat shock protein 27 enhances the inhibitory effect of influenza A virus NS1 on the expression of interferon-beta.
- Author:
Zheng LI
1
;
Xiaoling LIU
;
Zhendong ZHAO
;
Wenjun LIU
Author Information
1. CAS Key Laboratory of Pathogenic Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China.
- Publication Type:Journal Article
- MeSH:
Cell Line;
Gene Expression Regulation, Viral;
HEK293 Cells;
HSP27 Heat-Shock Proteins;
genetics;
pharmacology;
Humans;
Influenza, Human;
genetics;
immunology;
Interferon-beta;
antagonists & inhibitors;
metabolism;
Viral Nonstructural Proteins;
genetics;
pharmacology
- From:
Chinese Journal of Biotechnology
2012;28(10):1205-1215
- CountryChina
- Language:Chinese
-
Abstract:
Heat shock protein 27 (HSP27) is a member of the small heat shock proteins (sHSP) and has multiple functions, it also plays an important role in the life cycle of some viruses. To investigate the regulatory effect of HSP27 during influenza virus infection, we cloned and expressed human HSP27 in both prokaryotic and eukaryotic cells, and demonstrated that HSP27 interacted with influenza A virus NS1 protein both in vivo and in vitro. Luciferase assay showed that HSP27 inhibited the expression of interferon-beta (IFN-beta) in infected cells, and independent of its phosphorylation. Moreover, HSP27 enhanced the inhibitory effect of NS1 on the expression of IFN-beta. Further analysis indicated that HSP27 exerted the inhibitory effect probably through influencing MDA5 of the RIG-I like helicase (RLH) pathway. The results suggested that HSP27 play a role in the innate immunity of infected cells, contributed to our understanding of the regulatory effect of host factors during influenza virus infection.
