The distribution of gene polymorphisms in the intron 2 and exon 2 of interleukin-1 receptor antagonist and their correlation with the serum lipoprotein level.
- Author:
Pu XU
1
;
Yan LI
;
Ping-An ZHANG
;
Xiao-Yan LI
;
Geng-Shan LI
Author Information
- Publication Type:Journal Article
- MeSH: Adolescent; Adult; Aged; Aged, 80 and over; Exons; genetics; Female; Gene Frequency; Genetic Predisposition to Disease; Humans; Hyperlipidemias; blood; genetics; Interleukin 1 Receptor Antagonist Protein; Introns; genetics; Lipoproteins; blood; Male; Middle Aged; Minisatellite Repeats; Polymorphism, Genetic; Polymorphism, Restriction Fragment Length; Polymorphism, Single Nucleotide; Sialoglycoproteins; genetics
- From: Chinese Journal of Epidemiology 2004;25(6):522-526
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo study the distribution of variable numbers of tandem repeat (VNTR) polymorphism in the intron 2 and the single nucleotide polymorphism (SNP) at position +8006 in the exon 2 of IL-1RN in healthy Chinese Han Population of Wuhan province and to analyze their correlation with the serum lipoprotein level.
METHODSIL-1RN (VNTR) and IL-1RN (+8006) polymorphisms were detected by PCR and PCR-RFLP methods in 251 healthy Chinese Han Population of Wuhan, and the levels of serum lipoprotein, IL-1 and IL-1Ra were inspected simultaneously.
RESULTSIn IL-1RN (VNTR), allele I appeared most common, then allele II, and allele IV was rare. At the position of IL-1RN (+8006), allele T was most commonly seen followed by allele C. Allele II of IL-1RN (VNTR) always existed with allele C of IL-1RN (+8006). The levels of serum lipoprotein, IL-1 and IL-1Ra were not different among the different genotypes of the two polymorphisms.
CONCLUSIONThere were two gene polymorphisms in the intron 2 and exon 2 of IL-1RN, which were not correlated with the levels of serum lipoprotein, IL-1 and IL-1Ra. However, there seemed to be a linkage disequilibrium between IL-1RN (VNTR) and IL-1RN (+8006).
