Reprogramming of adult human neural stem cells into induced pluripotent stem cells.
- Author:
Li-Qian XIE
1
;
Hua-Ping SUN
;
Tian WANG
;
Hai-Liang TANG
;
Pu WANG
;
Jian-Hong ZHU
;
Zheng-Wei YAO
;
Xiao-Yuan FENG
Author Information
- Publication Type:Journal Article
- MeSH: Cell Differentiation; genetics; physiology; Cells, Cultured; Cellular Reprogramming; genetics; physiology; Humans; Immunohistochemistry; Induced Pluripotent Stem Cells; cytology; metabolism; Kruppel-Like Transcription Factors; metabolism; Neural Stem Cells; cytology; metabolism; Octamer Transcription Factor-3; metabolism; Proto-Oncogene Proteins c-myc; metabolism; Reverse Transcriptase Polymerase Chain Reaction; SOXB1 Transcription Factors; metabolism
- From: Chinese Medical Journal 2013;126(6):1138-1143
- CountryChina
- Language:English
-
Abstract:
BACKGROUNDSince an effective method for generating induced pluripotent stem cells (iPSCs) from human neural stem cells (hNSCs) can offer us a promising tool for studying brain diseases, here we reported direct reprogramming of adult hNSCs into iPSCs by retroviral transduction of four defined factors.
METHODSNSCs were successfully isolated and cultured from the hippocampus tissue of epilepsy patients. When combined with four factors (OCT3/4, SOX2, KLF4, and c-MYC), iPSCs colonies were successfully obtained.
RESULTSMorphological characterization and specific genetic expression confirmed that these hNSCs-derived iPSCs showed embryonic stem cells-like properties, which include the ability to differentiate into all three germ layers both in vitro and in vivo.
CONCLUSIONOur method would be useful for generating human iPSCs from NSCs and provide an important tool for studying neurological diseases.
