Expression and clinical significance of Ephrin-A1 in primary hepatocellular carcinoma.
- Author:
Gong ZHANG
1
;
Shui-Jun ZHANG
;
Yong-Fu ZHAO
;
Yang WU
;
Zhen LI
;
Jun-Xia WANG
Author Information
- Publication Type:Journal Article
- MeSH: Carcinoma, Hepatocellular; genetics; metabolism; pathology; Cell Differentiation; Ephrin-A1; genetics; metabolism; Gene Expression Regulation, Neoplastic; Humans; Immunohistochemistry; Liver Neoplasms; genetics; metabolism; pathology; Neoplasm Metastasis; RNA, Messenger; genetics; metabolism; Reverse Transcriptase Polymerase Chain Reaction
- From: Chinese Journal of Surgery 2007;45(7):499-502
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the expression and clinical significance of Ephrin-A1 mRNA and protein expression level in hepatocellular carcinoma.
METHODSReverse transcription polymerase chain reaction (RT-PCR) and immunohistochemistry technique were used to detect the expression of the Ephrin-A1 mRNA and protein of 40 cases of hepatocellular carcinoma and their corresponding para-cancerous tissues and 10 cases of normal liver tissues. The relationships with its clinical pathology characters were analyzed.
RESULTSThe mRNA of Ephrin-A1 was expressed in all of the 40 cases of hepatocellular carcinoma and their corresponding para-cancerous tissues and 10 cases of normal liver tissues. Semiquantitative analysis showed that the mRNA expression level of Ephrin-A1 in hepatocellular carcinoma (0.5413 +/- 0.1527) was greater than that in corresponding para-cancerous tissues (0.3895 +/- 0.0549, P < 0.05) and normal liver tissues (0.3770 +/- 0.1055, P < 0.05); but between corresponding para-cancerous tissues (0.3895 +/- 0.0549) and normal liver tissues (0.3770 +/- 0.1055), the mRNA expression level had no significant difference (P > 0.05). The positive rates of Ephrin-A1 protein were 20% (2/10) in normal tissues, 35% (14/40) in para-cancerous tissues and 62% (25/40) in hepatocellular carcinoma tissues, respectively; the protein expression level of Ephrin-A1 was gradually rising (chi(2) = 14.762, P < 0.05). The overexpression of Ephrin-A1 protein was correlated with histological differentiation, tumor thrombi in portal vein and lymph node metastasis (P < 0.05).
CONCLUSIONSThe overexpression of Ephrin-A1 protein is correlated with histological differentiation, the lymph node metastasis and tumor thrombi in portal vein. It indicates that Ephrin-A1 may play an important role in the malignancy transformation, invasion progression and metastasis of hepatocellular carcinoma.
