TNF-alpha induced reversal of multidrug resistance in human hepatocellular carcinoma cells.
- Author:
Qi WANG
1
;
Xiao-ping CHEN
;
Shan HAI
;
Gao-peng LI
;
Jian GUAN
;
Bi-xiang ZHANG
;
Wan-guang ZHANG
Author Information
- Publication Type:Journal Article
- MeSH: ATP-Binding Cassette, Sub-Family B, Member 1; genetics; metabolism; Antineoplastic Agents; pharmacology; Apoptosis; drug effects; Carcinoma, Hepatocellular; genetics; metabolism; pathology; Cell Line, Tumor; Doxorubicin; pharmacology; Drug Resistance, Multiple; drug effects; Drug Resistance, Neoplasm; drug effects; Humans; Liver Neoplasms; genetics; metabolism; pathology; PPAR alpha; genetics; metabolism; RNA, Messenger; genetics; metabolism; Reverse Transcriptase Polymerase Chain Reaction; Tumor Necrosis Factor-alpha; administration & dosage; pharmacology
- From: Chinese Journal of Surgery 2007;45(9):602-604
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the reversal of multidrug resistance in the cell line HepG2/ADM induced by TNF-alpha.
METHODSHepG2/ADM cells were incubated with different concentrations of TNF-alpha (100, 500 and 2500 U/ml) for 72 h. Real-time PCR was performed to compare the mRNA levels of MDR1 with PPAR-alpha in the different concentrations of TNF-alpha treated cells. The Annexin V assay was used to check cell apoptosis induced by 0.5 mg/L adriamycin. Rhodamine 123 efflux assay and MTT assay were used to study P-gp activity and drug resistance in each group, respectively.
RESULTSTNF-alpha could induce down-regulation of MDR1 and up-regulation of PPAR-alpha. Meanwhile, it could enhance cell cytotoxicity and cell apoptosis induced by 0.5 mg/L adriamycin.
CONCLUSIONSTNF-alpha could partially reverse the multidrug resistance of HepG2/ADM cells by down-regulating the expression of MDR1 and up-regulating the expression of PPAR-alpha.
