Cytotoxic effect of thermo-chemotherapy with cisplatin on osteosarcoma OS-732 cell line.
- Author:
Shuang-lin WAN
1
;
Jian ZHANG
;
Di-sheng YANG
;
Shun-wu FAN
Author Information
- Publication Type:Journal Article
- MeSH: Antineoplastic Agents; pharmacology; Apoptosis; drug effects; Bone Neoplasms; pathology; therapy; Cell Line, Tumor; Cisplatin; pharmacology; Combined Modality Therapy; DNA Damage; Flow Cytometry; Humans; Hyperthermia, Induced; Osteosarcoma; pathology; therapy
- From: Journal of Zhejiang University. Medical sciences 2003;32(5):427-432
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo observe the cytotoxic effect of thermo-chemotherapy with cisplatin on osteosarcoma OS-732 cell line and to explore its mechanism.
METHODSThe osteosarcoma OS-732 cell line was treated with different temperature, cisplatin alone and 43 degrees C +cisplatin for 1 h, respectively and the in vitro cytotoxic effect was observed with MTT assay. The cell cycle and the apoptotic rate were analyzed with flow cytometry (FCM); the cellular apoptosis was observed also with electron microscope.
RESULTThe cytotoxicity index increase markedly as the temperature elevated or the concentration of cisplatin increased. While treated with 43 degrees C hyperthermia and cisplatin simultaneously, the cytotoxicity index increased to 72.37%;the cell cycle of the treated OS-732 cells line was changed with marked increase in S phase and decreasing in G(2)/M phase. The apoptotic rate increased markedly with the highest of 56.47%. Electron microscope showed the characteristic apoptotic alterations.
CONCLUSIONHyperthermia with cisplatin enhance cytotoxicity on osteosarcoma OS-732 cell line and the induced cell apoptosis may be one of the mechanisms of enhanced cytotoxicity by thermo-chemotherapy.
