Correlation between basic expression level of NMDA receptor NR1 subunit protein in hippocampus and learning ability in rats.
- Author:
Shu-jun XU
1
;
Hai-qing SHEN
;
Zhong CHEN
;
Li-jun ZHU
;
Zhao-yang ZHU
;
Jian-hong LUO
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Hippocampus; chemistry; physiology; Immunoblotting; Learning; Male; Maze Learning; Rats; Rats, Sprague-Dawley; Reaction Time; Receptors, N-Methyl-D-Aspartate; analysis; physiology
- From: Journal of Zhejiang University. Medical sciences 2003;32(6):465-469
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the relationship between basic expression level of NMDA receptor NR1 subunit protein in hippocampus and learning ability of rats.
METHODSUsing a novel-object recognition model and Morris water maze,the novel-object recognition ability and spatial memory of SD rats were ranked, and grouped as the high (top 20 %) and the low (bottom 20%), respectively. NR1 subunit protein levels in hippocampus were measured by quantitative immunoblotting with NR1 subunit specific antibody.
RESULTThe level of NR1 subunit protein in hippocampus in the high novel-object recognition ability group was 60% (P<0.01), higher than that in the low one, and in the high spatial memory group it was 45.4 % (P<0.05), higher than that in the low one, respectively.
CONCLUSIONThe basic expression level of NR1 subunit protein in hippocampus is related to novel-object recognition ability and spatial memory of rats.
