Sequence detection of HBV-DNA P and C region in HIV/HBV superinfection subjects with drug resistance to HAART.
- Author:
Biao ZHU
1
;
Nan-ping WU
;
Armin BADER
;
Norbert BROCKMEYER
Author Information
- Publication Type:Journal Article
- MeSH: Antiretroviral Therapy, Highly Active; Base Sequence; DNA, Viral; chemistry; Drug Resistance, Viral; HIV Infections; drug therapy; virology; Hepatitis B; drug therapy; virology; Hepatitis B virus; genetics; Humans; Mutation
- From: Journal of Zhejiang University. Medical sciences 2003;32(6):507-509
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo further study the resistance of HBV to high activity antiretrovirus therapy(HAART).
METHODSHBV-DNA was quantitatively detected by real-time PCR in 36 HIV/HBV superinfection subjects, C region and P region HBV-DNA in high copies HBV-DNA subjects were detected by routine PCR, PCR products were purified and sequenced and compared with the HBV international Genbank using BLSAT softy ware.
RESULTHBV-DNA was positive in 4 of 36 patients (11.1%) and another 3 had low copies(<10(4)copies/ml), one had a high HBV-DNA copies (10(7)copies/ml). It's HBV-DNA C region sequence had mutation on 2 sites (nt 2412 T/C; nt 2413 T/C) and 1 mutation P region (nt 741 A/G, also YMDD/YVDD) compared with HBV international Genbak reference sequence.
CONCLUSIONThe HBV resistance to HAART may be related with multiple genetic mutations in the C and P regain of HBV-DNA.
