Determination of amlodipine in CYP3A4 cDNA-expressed cells by HPLC.
- Author:
Yong-jiang WU
1
;
Yun-xue PAN
;
Su ZENG
Author Information
- Publication Type:Journal Article
- MeSH: Amlodipine; analysis; metabolism; Calcium Channel Blockers; analysis; Chromatography, High Pressure Liquid; Cytochrome P-450 CYP3A; Cytochrome P-450 Enzyme System; genetics; physiology; Humans; Transfection
- From: Journal of Zhejiang University. Medical sciences 2003;32(6):510-513
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo establish a RP-HPLC method for the determination of amlodipine after metabolism by cytochrome P450 cDNA-expressed cells.
METHODSThe determination was performed on a C(18) reversed phase column with a mobile phase composed of acetonitrile phosphates buffer (45:55, v/v, pH 4.5) with UV detection (lambda250nm). Propranolol was used as the internal standard.
RESULTThe standard curve was linear over the concentration range of 0.2 - 30.0 microg/ml (r=0.9993), and the limits of determination was 20 ng/ml (S/N >or=3), the limits of quantity was 0.2 microg/ml (recovery 104.0%, RSD 11.4%, n=5). The recovery for this assay was (98.2+/-2.4)%, precision for inter-assay and intra-assay was <10 % and 6 %, respectively.
CONCLUSIONThe HPLC method established is simple, accurate and suitable for the determination of amlodipine in cytochrome p450 cDNA-expressed cells.
