Suppression of nitric oxide on cytotoxicity of Lomustine in glioma cell line BT- 325.
- Author:
Run-lin GAO
1
;
Zeng-liang CHEN
Author Information
- Publication Type:Journal Article
- MeSH: Antineoplastic Agents, Alkylating; pharmacology; Cell Line, Tumor; Drug Resistance, Neoplasm; Glioma; drug therapy; pathology; Humans; Interleukin-1; pharmacology; Lipopolysaccharides; pharmacology; Lomustine; pharmacology; NG-Nitroarginine Methyl Ester; pharmacology; Nitric Oxide; physiology
- From: Journal of Zhejiang University. Medical sciences 2003;32(6):519-528
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the effect of nitric oxide (NO) on cytotoxicity of Lomustine (CCNU) in vitro.
METHODSCCNU was used to treat human glioma cell line BT-325 with different concentration of cytokines or NO donors, NO levels was measured by Griess assay and cell survival was evaluated by MTT assay.
RESULT(1) Pretreatment with IL-1 beta and LPS markedly suppressed CCNU cytotoxicity in BT-325 cells with a significant increase in NO production (P<0.05). This function could be inhibited by L-NAME. (2) DETA NONOate suppressed cytotoxicity of CCNU to BT -325 cells in a dose-dependent manner (P<0.05). (3) CCNU co-cultured with SNAP for 24 h showed higher cytotoxic to BT-325 cells(P<0.05).
CONCLUSIONNO partly suppresses cytotoxicity of Lomustine, which might be associated with chemoresistance of BT-325 cells against CCNU in vitro. NO can also slow the degradation of CCNU in water solution.
