Effects of sodium hyaluronate on growth and adhesion of two human colorectal cancer cell lines in vitro.
- Author:
Lin-bo WANG
1
;
Shu-duo XIE
;
Qing-hua DONG
;
Ling-ling YU
;
Wei-feng LAO
;
Xiang-yang SONG
;
Chao HE
Author Information
- Publication Type:Journal Article
- MeSH: Cell Adhesion; drug effects; Cell Division; drug effects; Cell Line, Tumor; Colorectal Neoplasms; pathology; Humans; Hyaluronan Receptors; analysis; Hyaluronic Acid; pharmacology
- From: Journal of Zhejiang University. Medical sciences 2003;32(6):525-528
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the effects of sodium hyaluronate on the growth and adhesion of colorectal cancer cells.
METHODSHuman colorectal cancer cell lines SW620 and Colo205 were treated with sodium hyaluronate (25 -2,500 microg/ml), and cancer cell proliferation was measured by MTT assay in vitro. Flow-cytometric analysis was applied to detect expression of CD44 on SW620 and Colo205 cells.
RESULTIn vitro sodium hyaluronate enhanced proliferation of Colo205 cells, but it had no appreciable effect on SW620 growth under the same doses, Meantime, CD44 expression on cancer cells decreased compared with controls.
CONCLUSIONIn vitro sodium hyaluronate has different effects on growth of different colorectal cancer cell lines, but can inhibit CD44 expression of colorectal cancer cells and influence their ability of adhesion.
