Direct resolution of calcium folinate stereoisomers using a bovine serum albumin chiral HPLC column.
- Author:
Shui-Jie SHEN
1
;
Su ZENG
Author Information
- Publication Type:Journal Article
- MeSH: Chromatography, High Pressure Liquid; Hydrogen-Ion Concentration; Leucovorin; chemistry; metabolism; Serum Albumin, Bovine; metabolism; Stereoisomerism
- From: Journal of Zhejiang University. Medical sciences 2004;33(1):11-14
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo establish a direct and fast method separating calcium levofolinate and calcium dextrofolinate in a bovine serum albumin stationary phase chiral column.
METHODSUsing EC150/4 RESOLVOSIL BSA-7(150 mm x 4 mm) chiral separation column, with 0.20 mol/L, pH=5.0 phosphate buffer as mobile phase HPLC method was performed to separate calcium folinate enantiomers.
RESULTThe capacity factor and resolution of the two calcium folinate enantiomers were greatly affected by mobile phase buffer concentration,pH and the column temperature. And the retention time of calcium levofolinate and calcium dextrofolinate were 18.5 min and 22.6 min, respectively. The resolution, R(s)=1.49.
CONCLUSIONCalcium folinate enantiomers are separated successfully using this method.
