Interaction between ipriflavone and other drugs in metabolism in vitro.
- Author:
Xiao-yu HU
1
;
Tong-wei YAO
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Drug Interactions; Estradiol; metabolism; Female; Isoflavones; metabolism; Propafenone; metabolism; Propranolol; metabolism; Rats; Rats, Sprague-Dawley
- From: Journal of Zhejiang University. Medical sciences 2004;33(1):21-23
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the metabolism-based interaction between ipriflavone(IP) and other drugs in vitro.
METHODSIP and propafenone or estradiol, or other drugs were co-incubated with rat liver microsome induced by beta-naphthoflavone. The concentrations of residual substrate in microsomal incubates were determined by HPLC.
RESULTThe metabolism of propafenone (10 microg/ml) incubated with 2 microg/ml or 50 microg/ml IP was markedly inhibited compared with control group(P<0.01). While the metabolism of 10 microg/ml of estradiol incubated with 10 microg/ml and 100 microg/ml IP showed no marked inhibition. When IP (20 microg/ml) was incubated with 0.5 microg/ml propranolol, 0.5 microg/ml propafenone or 5.0 microg/ml estradiol respectively the metabolic activity of IP was markedly inhibited compared with control group (P<0.05 P<0.05 P<0.02 respectively).
CONCLUSIONThe results indicate that there is an interaction between ipriflavone and propafenone during in vitro metabolism, and there might also be clinical significance.