HPLC determination of zolmitriptan and its related substances.
- Author:
Yun-zhen HU
1
;
Tong-wei YAO
;
Xiang-jun WANG
Author Information
- Publication Type:Journal Article
- MeSH: Chromatography, High Pressure Liquid; Oxazolidinones; analysis; Tryptamines
- From: Journal of Zhejiang University. Medical sciences 2004;33(1):37-40
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo develop an analytical method and quality control for determination of zolmitriptan and related substances.
METHODSZolmitriptan and related substances were separated and determined on a shimadzu CLC-C(8) column (150 mm x 6 mm, 10 microm) with a mobile phase of acetonitrile-10 mmol/L phosphate buffer (25:75 pH 7.5) and a flow-rate of 1 ml/min; the UV-VIS detector was operated at 229 nm.
RESULTThe limit of detection for the related substances was 0.5 ng on the zolmitriptan basis (S/N >3). Linear calibration curve was gene rated from 4 - 40 microg/ml with a correlation coefficient of 0.9999. The recovery rate of zolmitriptan was 99.1% with a standard deviation of 0.2%. The results of HPLC method were consistent with those of nonaqueous titration method.
CONCLUSIONHPLC method is a rapid sensitive and accurate method for the determination of zolmitriptan and its related substances.
