Expression of transforming growth factor-beta1 in renal fibrosis of human mesengial proliferative glomerulonephritis.
- Author:
Jiang LIU
1
;
Hua LIANG
;
Chang-sheng LIU
;
Jin-kang JIA
;
Hong-lei WENG
;
Jia-xi CHEN
Author Information
- Publication Type:Journal Article
- MeSH: Collagen; analysis; Fibrosis; Glomerulonephritis, Membranoproliferative; metabolism; pathology; Humans; Immunohistochemistry; Kidney; pathology; Transforming Growth Factor beta; analysis; Transforming Growth Factor beta1
- From: Journal of Zhejiang University. Medical sciences 2004;33(1):46-54
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo explore the possible effect of transforming growth factor-beta(1) (TGF -beta(1)) on the development of renal fibrosis in human mesengial proliferative glomerulonephritis (MsPGN).
METHODSImmunohistochemistry method, sirius red staining polarization microscopy and the computer imaging analysis system were used to detect the expression of TGF-beta(1), the distribution of collagen I, collagen III and collagen IV.
RESULTIn MsPGN with renal fibrosis, collagen IV was increased markedly,and collagen I and collagen III appeared in the expanded mesengial matrix abnormally. Collagen III and collagen IV were increased markedly in tubulointerstitium. TGF-beta(1) expression was positively correlated with the expression of collagen I, collagen III and collagen IV in tubulointerstitium (r=0.82 0.92,P<0.01), and negatively correlated with I/III, I/IV and III/IV (r=-0.83,-0.92, P<0.001).
CONCLUSIONAbnormal increase of TGF-beta(1) may be one of the important factors associated with glomerular sclerosis and tubulointerstitial fibrosis through the increment and abnormal distribution of collagen I, collagen III and collagen IV.
