TGF-beta1mRNA levels in renal cortex and peripheral blood mononuclear cells of diabetic rats.
- Author:
Hong LI
1
;
Fen-ping ZHENG
;
Zhe ZHANG
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Diabetes Mellitus, Experimental; metabolism; Female; Kidney Cortex; metabolism; Leukocytes, Mononuclear; metabolism; Male; RNA, Messenger; analysis; Rats; Rats, Sprague-Dawley; Streptozocin; Transforming Growth Factor beta; genetics; Transforming Growth Factor beta1
- From: Journal of Zhejiang University. Medical sciences 2004;33(1):51-54
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the effect of hyperglycemia and pioglitazone on TGF-beta(1) gene expression in peripheral blood mononuclear cells (PBMC) and renal cortex, and the correlation of TGF-beta(1)mRNA levels between PBMC and renal cortex in STZ induced diabetic rats.
METHODSFifty-four Sprague-Dawley rats were randomly divided into three groups: 18 normal control rats (group C), 18 diabetic rats (group D) and 18 diabetic rats treated with pioglitazone (20 mg x kg(-1)x d(-1), group DP). Six rats from each group were sacrificed at 2, 4, 8 weeks. TGF-beta(1)mRNA levels of PBMC and renal cortex were examined by RT-PCR+Slit hybridization analysis.
RESULTTGF-beta(1)mRNA level of renal cortex in group D was significantly higher than that in group C at each time point (P<0. 05); TGF-beta(1)mRNA level of PMBC in group D was slightly higher than that of group C at 4 weeks, and significantly higher at 8 weeks (P=0.01). There was positive correlation of TGF -beta(1)mRNA level between PBMC and renal cortex before (r=0.83, P=0.02) and after pioglitazone treatment at 8 weeks (r=0.82, P=0.03).
CONCLUSIONTGF-beta(1)mRNA level of PBMC may reflect the change of TGF-beta(1) gene expression of renal cortex in diabetic rats.
