Effect of fragile histidine triad gene on apoptosis of MGC-803 gastric cancer cells.
- Author:
Mei KONG
1
;
Wei-zhe CHEN
;
Zhi CHEN
;
Min-wei LI
Author Information
- Publication Type:Journal Article
- MeSH: Acid Anhydride Hydrolases; Apoptosis; Cell Line, Tumor; Flow Cytometry; Genes, Tumor Suppressor; Humans; Immunohistochemistry; Neoplasm Proteins; analysis; genetics; Stomach Neoplasms; genetics; pathology; prevention & control; Transfection
- From: Journal of Zhejiang University. Medical sciences 2004;33(2):133-137
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the effect of fragile histidine triad (FHIT) gene on the apoptosis of gastric cancer cells.
METHODSFHIT gene was transfected into gastric cancer cell line MGC-803 by liposome. Western blot and immunohistochemical assay were employed to detect the expression of FHIT. Apoptosis and cell cycle were analyzed by flow cytometry (FCM). Morphological changes were observed by light and electron microscope.
RESULTSStable expression of FHIT protein in transfected MGC-803 cells was obtained. The rates of G0/G1 phase and apoptosis cells were significantly higher in FHIT-transfected MGC-803 cells than those in control cells (64.4%compared with 49.5%and 32.0%compared with 12.2%, respectively).
CONCLUSIONThe results indicate that FHIT gene might be involved in the apoptosis of gastric cancer cells.
