Regulation of CD3, CD4 and CD8 expressions on PMA-activated human peripheral T cells.
- Author:
Jian-hua MAO
1
;
Zhi-min CHEN
;
Yong-min TANG
;
Li LIANG
;
Li-zhong DU
;
Yang ZHANG
Author Information
- Publication Type:Journal Article
- MeSH: CD3 Complex; blood; CD4 Antigens; blood; CD8 Antigens; blood; Child; Humans; Interferon-gamma; biosynthesis; Interleukin-4; biosynthesis; Lymphocyte Activation; drug effects; T-Lymphocytes; chemistry; drug effects; Tetradecanoylphorbol Acetate; pharmacology; Th1 Cells; immunology; Th2 Cells; immunology
- From: Journal of Zhejiang University. Medical sciences 2004;33(2):155-159
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the effect of mitogen Phorbol 12-myristate 13-Acetate (PMA) on CD3, CD4 and CD8 expression of human T-lymphocytes.
METHODSPeripheral blood mononuclear cells from 37 blood samples stimulated in vitro with PMA at different concentrations (2,5,10,20 and 50 ng/ml for 4 hours) and time (10 ng/ml for 2,4 and 6 hours) were analyzed by 4-color flow cytometry (FCM).
RESULTSUnder different PMA stimulation protocols,significant CD4 down-regulation was observed,which was negatively correlated with intracellular cytokine secretion (r= 0.601,P<.001), except for PMA stimulation at 10 ng/ml for 2-hours which showed no significant intracellular cytokine secretion. The expressions of CD3 and CD8 molecules after PMA activation were not significantly affected as compared with pre-activation. Among CD3 positive T lymphocytes, CD4/CD8 double-negative cells only account for 5.52%.
CONCLUSIONPMA has a significant down-regulation effect on CD4 molecules of Th cells, without altering the CD3 and CD8 expression. For quantitative analysis of Th1/Th2 variation, indirect method such as CD3(+)CD8( ) T cells can be used to define CD4(+) Th cells stimulated by PMA in the future.
