Expression of PCNA and Bcl-2 in the traumatic brains implanted with embryonic brain tissue in rats.
- Author:
Hua HU
1
;
Wei-ming FU
;
Xiu-jue ZHENG
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Brain Injuries; metabolism; surgery; Brain Tissue Transplantation; Female; Fetal Tissue Transplantation; Proliferating Cell Nuclear Antigen; analysis; Proto-Oncogene Proteins c-bcl-2; analysis; Rats
- From: Journal of Zhejiang University. Medical sciences 2004;33(2):174-176
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the expression of PCNA and Bcl-2 in the traumatic brain area transplanted with embryonic brain tissue in rats.
METHODSThe cerebral contusion of rats was induced by dropping weight. The homogenates of embryonic brain tissue were transplanted into the traumatic brain area two weeks after injury. All rats were sacrificed 6 weeks after injury (4 weeks after transplantation), and their brains were examined histologically. The expressions of PCNA and Bcl-2 in the brains were analyzed by immunohistochemical methods.
RESULTSThe histology of brain presented the capillary and glia proliferation, especially in the transplantation group. No significant difference was found in the expression of PCNA between two groups. However, Bcl-2 was overexpressed in the transplantation group.
CONCLUSIONThe transplantation of the embryonic brain tissue enhances the expression of Bcl-2, which may play a neuroprotective role following traumatic brain injury.
