Reversing effect of histamine on neuron death induced by N-methyl-D-aspartate.
- Author:
Hai-bin DAI
1
;
Zhong CHEN
;
Yu-wen HUANG
;
Qiu-fu GE
;
Zhong-miao ZHANG
;
Er-qing WEI
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Cell Death; drug effects; Cells, Cultured; Dose-Response Relationship, Drug; Histamine; pharmacology; N-Methylaspartate; toxicity; Neurons; drug effects; Neuroprotective Agents; pharmacology; Rats; Rats, Sprague-Dawley; Receptors, Histamine H1; physiology; Receptors, Histamine H2; physiology
- From: Journal of Zhejiang University. Medical sciences 2004;33(3):189-192
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo determine the effect of histamine on N-methyl-D-aspartate (NMDA) induced neuron death and to elucidate its mechanism.
METHODSThe primary cortical cell culture was adopted. Neuron morphology and MTT assay were used to evaluate the drugs effects.
RESULTHistamine at doses of 10(-4) 10(-6) 10(-7) 10(-8) mol/L reversed the neuron death induced by NMDA (50 micromol/L) for 3 h. The protection of histamine peaked at doses of 10(-4) mol/L and 10(-7)mol/L. The effect of histamine of 10(-7) mol/L was reversed only by cimetidine an H(2)receptor antagonist. However, the effect of histamine of 10(-4) mol/L was reversed only by pyrilamine but not cimetidine.
CONCLUSIONHistamine could reduce neuron death induced by NMDA; its protection at a low dose might be mediated by H(2)receptor, and at a high dose by H(1)receptor.
