Effects of pentylenetetrazol at subconvulsant and convulsant dose on NMDA receptor subunits in rats.
- Author:
Li-jun ZHU
1
;
Min-zhen WANG
;
Xiao-hua WU
;
Shu-jun XU
;
Jian-hong LUO
;
Zhong CHEN
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Cerebral Cortex; chemistry; Dose-Response Relationship, Drug; Male; Pentylenetetrazole; administration & dosage; Rats; Rats, Sprague-Dawley; Receptors, N-Methyl-D-Aspartate; analysis; Seizures; chemically induced; metabolism
- From: Journal of Zhejiang University. Medical sciences 2004;33(3):213-224
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the expression of N-methyl-D-asparate (NMDA) receptor subunit proteins after administration of different doses of pentylenetetrazol (PTZ).
METHODSAfter ip injection of a subconvulsant (35 mg/kg) and convulsant (50 mg/kg) dose of PTZ, the rats were decapitated at different time points. The levels of cortical NR1 NR2A and NR2B subunit proteins were detected by immunoblotting.
RESULT35 mg/kg PTZ and 50 mg/kg PTZ elicited different behavioral changes (P<0.001). The NR2A subunit in the cortex significantly increased 1 h after PTZ injection (P<0.05). For the 50 mg/kg group, both the NR2A and NR2B subunits proteins increased at 1 h in the cortex and then decreased; the protein levels returned to normal after 48 h. However, NR1 subunit had no changes.
CONCLUSIONThe NR2 subunit is involved in PTZ-induced seizure.
