Expression of matrix metalloproteinase-2 and-9 in kidney of diabetic rats.
- Author:
Feng-qin DONG
1
;
Hong LI
;
Wei-min CAI
;
Jun TAO
;
Fen-ping ZHEN
;
Zhe ZHANG
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Collagen Type IV; analysis; Diabetes Mellitus, Experimental; enzymology; pathology; Female; Immunohistochemistry; Kidney; enzymology; pathology; Male; Matrix Metalloproteinase 2; analysis; Matrix Metalloproteinase 9; analysis; Rats; Rats, Sprague-Dawley; Transforming Growth Factor beta; analysis; Transforming Growth Factor beta1
- From: Journal of Zhejiang University. Medical sciences 2004;33(3):245-249
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the expression of matrix metalloproteinase-2 (MMP-2), matrix metalloproteinase-9(MMP-9), transforming growth factor beta(1)(TGF-beta(1)) and IV-collagen (C-IV) in kidneys of diabetic rats.
METHODSRat diabetic model was induced by streptozotocin (70 mg/kg), and kidneys were examined pathologically and the expressions of MMP- 2, MMP-9, TGF-beta(1) and C-IV were studied by immunohistochemistry. The results were analyzed by imaging quantitative analysis technique.
RESULTImmunoreactive MMP-2 and MMP-9 were mainly expressed in the mesangial cells, endothelial cells, parietal layer of Bowman's capsule and tubular cells. The expression of MMP-2 was significantly weaker in the glomeluri of diabetic rats than that of the control animals (P<0.05), while the expression of TGF-beta(1) and C-IV in the glomeluri of diabetic rats was significantly stronger than that of the controls (P%lt;0.05). The expression of MMP-9 didn't show significant different in glomeluri of the two groups (P>0.05).
CONCLUSIONExpression of MMP-2 in glomeluri is decreased in diabetic rats, which may be related to the increased TGF-beta(1) and in turns promote the accumulation of C-IV.
