Effect of homocysteine on NF-kappaB activity in cultured rat vascular smooth muscle cells.
- Author:
Liang-rong ZHENG
1
;
Jian-hua ZHU
;
Li ZHANG
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Arteriosclerosis; etiology; Electrophoretic Mobility Shift Assay; Female; Homocysteine; pharmacology; Male; Muscle, Smooth, Vascular; cytology; metabolism; Myocytes, Smooth Muscle; metabolism; NF-kappa B; metabolism; Rats; Rats, Sprague-Dawley
- From: Journal of Zhejiang University. Medical sciences 2004;33(3):255-268
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the effect of homocysteine (Hcy) on nuclear factor-kappaB (NF-kappaB) activity in cultured rat vascular smooth muscle cells (VSMCs).
METHODSRat VSMCs were stimulated with 0.25 mmol/L Hcy. Cells were collected and nuclear protein was extracted at 30 min, 1 h and 2 h following stimulation. NF-kappaB activity was examined by electrophoretic mobility shift assay (EMSA).
RESULTBaseline NF-kappaB nuclear binding in VSMCs increased more than 1.76-fold at 30 min (P<0.01), 1.91-fold at 1h (P<0.01) and 1.84-fold at 2 h (P<0.01) following stimulation of Hcy.
CONCLUSIONHcy can enhance transient mobilization of N-kappaB in VSMCs, which suggests that some regulating effect of Hcy on VSMCs might be exerted through NF-kappaB activation pathway.
