Skin-like structure generated from implantation of hair follicle bulb cells into collagen/chitosan porous scaffolds in vitro.

Author: Xian-jie WU ¹ ; Zhong-fa LU ; Min ZHENG ; Yue-lan CHAO ; Sui-qing CAI ; Jian-guang ZHOU ; Lie MA ; Chang-you GAO
Author Information

1. Department of Dermatology, the Second Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou 310009, China.
Publication Type:Journal Article
MeSH: Animals; Chitin; analogs & derivatives; Chitosan; Collagen; Hair Follicle; cytology; Mice; Mice, Inbred C57BL; Regeneration; Skin; cytology; Tissue Engineering
From: Journal of Zhejiang University. Medical sciences 2004;33(4):281-286
CountryChina
Language:Chinese
Abstract:
OBJECTIVETo observe the skin regeneration after hair follicle bulb cells were implanted into collagen/chitosan porous scaffolds in vitro.
METHODSThe cultured dorsal hair follicle bulb cells of 4d-old C57BL/6J mice were implanted into collagen/chitosan porous scaffolds in vitro. The skin regeneration was observed.
RESULTThe skin-like structure was formed on the collagen/chitosan porous scaffolds where were cultured the hair follicle bulb cells before 4th passages.
CONCLUSIONThe skin-like structure is generated in vitro when early passages of cultured hair bulb cells are implanted into collagen/chitosan porous scaffolds.