Research on stability of melittin in different solvent systems.
- Author:
Ke-xin SHANG
;
Jie BAI
;
Hui-hao WU
;
Yang LU
;
Shou-ying DU
;
Chang YANG
;
Yan-ke CHENG
- Publication Type:Journal Article
- MeSH:
Animals;
Drug Stability;
Ethanol;
chemistry;
Hydrogen-Ion Concentration;
Melitten;
chemistry;
Rats;
Skin;
drug effects;
Solvents;
chemistry;
Temperature
- From:
China Journal of Chinese Materia Medica
2014;39(22):4324-4328
- CountryChina
- Language:Chinese
-
Abstract:
The stability of melittin in different solvents (water, deoxygenated water, physiological saline, PBS, 50% ethanol, ethanol, glycerol)was studied and the results showed that the stability of melittin is not influenced by light, temperature and pH in 50% ethanol, which melittin can be completed dissolved when compared with ethanol and glycerol, in such, 50% ethanol was chosen as solvent storage when measured content of melittin. Then the effect of different concentrations of PBS, the pH of PBS and rat skin ho- mogenates were tested, and the results showed that melittin was degraded rapidly at low concentration solution and low ionic strength. Increasing pH of PBS and rat skin homogenate can accelerate the degradation of melittin. These researches provide an experimental ba- sis for further study of melittin.
