Spectrum-effect relationship of Moutan cortex against lipopolysaccharide-induced acute lung injury.
- Author:
Ming-jie TANG
;
Yong-shan YE
;
Qi ZHANG
;
Jian LI
;
Hai-min LEI
;
Cheng-ke CAI
;
Qiang LI
- Publication Type:Journal Article
- MeSH:
Acetophenones;
chemistry;
pharmacology;
Acute Lung Injury;
chemically induced;
drug therapy;
Animals;
Bridged Bicyclo Compounds, Heterocyclic;
chemistry;
pharmacology;
Chromatography, High Pressure Liquid;
methods;
Drugs, Chinese Herbal;
chemistry;
pharmacology;
Gallic Acid;
chemistry;
pharmacology;
Glucosides;
chemistry;
pharmacology;
Lipopolysaccharides;
pharmacology;
Male;
Monoterpenes;
chemistry;
pharmacology;
Paeonia;
chemistry;
Plant Roots;
chemistry;
Rats;
Rats, Wistar;
Tandem Mass Spectrometry;
methods
- From:
China Journal of Chinese Materia Medica
2014;39(22):4389-4393
- CountryChina
- Language:Chinese
-
Abstract:
This research is to study the relationship between HPLC fingerprints of Moutan Cortex, Paeoniae Radix Rubra and Paeoniae Radix Alba and their activity on lipopolysaccharide-induced acute lung injury. HPLC fingerprints of each extract of Moutan Cortex,Paeoniae Radix Rubra and Paeoniae Radix Alba were established by an optimized HPLC-MS method. The activities of all samples against protein and tumor necrosis a factor were tested by the model of lipopolysaccharide-induced acute lung injury. The possible relationship between HPLC-MS fingerprints and the activitieswere deduced by the Partial least squares regression analysis method. Samples were analyzed by HPLC-MS/MS to identify the major peaks. The results showed that each sample had some effect on acute lung injury. Four components with a lager contribution rate of efficacy were calculated by the research of spectrum-effect relationship. Moutan Cortex exhibited good activity on acute lung injury, and gallic acid, paeoniflorin, galloylpaeoniflorin and paeonol were the main effective components.
