Inhibitory effects of ethyl acetate extract of Huanglian Jiedu decoction on hyphae development of Candida albicans.
- Author:
Tian-ming WANG
;
Yuan-yuan YAN
;
Gao-xiang SHI
;
Dan XIA
;
Jing SHAO
;
Chang-zhong WANG
- Publication Type:Journal Article
- MeSH: Acetates; Biofilms; drug effects; growth & development; Candida albicans; cytology; drug effects; genetics; growth & development; Down-Regulation; Drugs, Chinese Herbal; pharmacology; Fungal Proteins; genetics; Gene Expression Regulation, Fungal; drug effects; Hyphae; Medicine, Chinese Traditional; Microscopy, Fluorescence; Reverse Transcriptase Polymerase Chain Reaction
- From: China Journal of Chinese Materia Medica 2014;39(24):4834-4838
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the effects of ethyl acetate extract of Huanglian Jiedu decoction (EAHD) on hyphae development of Candida albicans.
METHODInverted microscope, fluorescence microscope, SEM were applied to inspect the Morphological change of C. albicans treated by EAHD at different concentrations. Solid agar plate was utilized to evaluate the colony morphology. Quantitative Real-ime PCR(qRT-PCR) was adopted to observe the expression of hyphae-specific genes such as HWP1, ALS3, UME6, CSH1, SUN41, CaPDE2.
RESULTEAHD with concentration of 312 and 1 250 mg . L-1 could inhibit formation of hyphae and colony morphology. The expression of HWP1, ALS3, UME6, CSH1 were downregulated 4. 13, 3. 64, 2. 46, 2. 75 folds ,while the expression of SUN41 were upregulated 7. 26 folds, CaPDE2 keep unchanged.
CONCLUSIONEAHD could inhibit formation of hyphae and colony morphologies of C. albicans through downregulating HWP1, ALS3, UME6 and CSH1.