Influence of Ginkgo biloba extract on proliferation of ACC-2 cell, Survivin and TIP30 gene expression in adenoid cystic carcinoma of lacrimal gland.
- Author:
Po NIU
;
Xin-xia ZHAO
;
Fei YAN
;
Yong-ming ZHOU
;
Peng JIAN
- Publication Type:Journal Article
- MeSH:
Apoptosis;
drug effects;
Apoptosis Regulatory Proteins;
metabolism;
Carcinoma, Adenoid Cystic;
drug therapy;
Cell Cycle;
Cell Line, Tumor;
Cell Proliferation;
drug effects;
Drugs, Chinese Herbal;
Gene Expression;
Ginkgo biloba;
chemistry;
Humans;
Lacrimal Apparatus;
drug effects;
Plant Extracts;
chemistry;
isolation & purification;
pharmacology
- From:
China Journal of Chinese Materia Medica
2014;39(24):4860-4864
- CountryChina
- Language:Chinese
-
Abstract:
Exploring the influence of extract of Ginkgo biloba (EGB) on the proliferation, apoptosis of ACC-2 cell in lacrimal adenoid cystic carcinoma and analyzing the influence of EGB on the gene expression of Survivin and TIP30 based on the levels of the gene and protein. ACC-2 cell in human with ACC of lacrimal gland disposed by EGB of different concentration was in vitro cultured. MTT method was used for cell proliferation detection. Annexin V/PI double-staining flow cytometer was used to detect cell apoptosis and cell cycle. Survivin and TIP30 gene expression together with protein expression were analyzed by RT-PCR and Western blotting. And it is indicated that EGB has inhibitory effect on the proliferation of ACC-2 cell in vitro. Furthermore, the dose-effect relationship was significant. Compared with the control group, it had statistical difference (P <0.01). The inhibitory concentration 50% (ICso) is 88 mg . L-1. By flow cytometer examination, it was indicated that EGB can gradually increase ACC-2 cell in G0-G1 stage and decrease it in G2-M and S stage. With the increase of dose, the apoptosis rate of ACC-2 cell obviously increased (P <0.05 or P <0.01). Both of the expression results of RT-PCR and Western hybrid proteins have showed that the concentration of EGB increased, it could be seen a significant decrease in Survivin gene expression (P <0.01). Meanwhile, the TIP30 gene expression got a significant increase. Therefore, EGB can effectively inhibit ACC-2 cell Survivin gene expression in human with adenoid cysistic carcinoma of larcrimal gland as well as promoting TIP30 gene expression, inducing the ACC-2 cell apoptosis and inhibiting tumor cell proliferation, which provided a certain theoretical and experimental basis for the application of Chinese herbal medicinal ingredient in the treatment of tumors.
