Overexpression and RNAi vectors built for key secondary metabolic pathway genes PAL, HMGR, PGT of Arnebia euchroma.
- Author:
Teng XIE
;
Yu-Zhong LIU
;
Sheng WANG
;
Tan LIU
;
Li-Ping KANG
;
Lan-Ping GUO
- Publication Type:Journal Article
- MeSH:
Boraginaceae;
genetics;
metabolism;
Genetic Vectors;
genetics;
metabolism;
Plant Proteins;
genetics;
metabolism;
RNA Interference;
Secondary Metabolism
- From:
China Journal of Chinese Materia Medica
2014;39(23):4538-4543
- CountryChina
- Language:Chinese
-
Abstract:
Arnebia euchroma is the main source for medicinal herb Zicao. and its most important component shikonin compounds have high medicinal and industrial value. This research is aimed to build overexpression vectors and RNAi vectors for key secondary metabolism genes of A. euchroma, and bulid platform for constructions of related transgenic lines using GATEWAY technology. To build genetic material based genetic research platform is to provide a great convenience for digging and functional verification of the genes on secondary metabolic pathway, and also to fill the gaps in transgenic research of A. euchroma. This study is also important for the cultivation of shikonin high-yielding strains of A. euchroma.
