Human decorin regulates proliferation and migration of human lung cancer A549 cells.
- Author:
Shuo LIANG
1
;
Jin-Fu XU
1
;
Wei-Jun CAO
2
;
Hui-Ping LI
1
;
Cheng-Ping HU
3
Author Information
- Publication Type:Journal Article
- MeSH: Cell Cycle; genetics; physiology; Cell Movement; genetics; physiology; Cell Proliferation; Cyclin D1; genetics; metabolism; Decorin; genetics; metabolism; Humans; Receptor, Epidermal Growth Factor; genetics; metabolism; Transforming Growth Factor beta; genetics; metabolism; Tumor Cells, Cultured
- From: Chinese Medical Journal 2013;126(24):4736-4741
- CountryChina
- Language:English
-
Abstract:
BACKGROUNDDecorin is a small leucine-rich proteoglycan and it plays an important role in regulation of cell growth and migration in various tumor cell lines. Decorin was found down-regulated in non-small cell lung cancer tissue and may be involved in regulation of lung cancer development.
METHODSIn this study, lentivirus-mediated RNA interference and over expression were employed to change the expression levels of decorin in lung cancer A549 cells. We tested the cell cycle of A549 cells and the expression of transforming growth factor (TGF)-β, cyclin D1, epidermal growth factor receptor (EGFR), P53, and P21.
RESULTSWe found that up-regulation of decorin could inhibit proliferation, block cell cycle at G1 and decrease invasive activity of A549 cells. Moreover, we also show that up-regulation of decorin induced significant decreases of TGF-β1, cyclin D1 expression, phosphorylation of EGFR, and increases of P53 and P21 expression. Opposite results were observed in A549 cells with down-regulation of decorin.
CONCLUSIONOur results suggest that decorin is a key regulator involved in proliferation and migration of A549 cells.
