Prevention of catheter-related Pseudomonas aeruginosa infection by levofloxacin-impregnated catheters in vitro and in vivo.

Ping Yan; Wei Liu; Jinliang Kong; Hong WU; Yiqiang Chen

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Prevention of catheter-related Pseudomonas aeruginosa infection by levofloxacin-impregnated catheters in vitro and in vivo.

Author: Ping YAN ¹ ; Wei LIU ² ; Jinliang KONG ¹ ; Hong WU ³ ; Yiqiang CHEN ⁴
Author Information

1. Guangxi Institute of Respiratory Diseases, First Affiliated Hospital of Guangxi Medical University, Nanning, Guangxi 530021, China.
2. School of Pharmaceutical Sciences, Guangxi Medical University, Nanning, Guangxi 530021, China.
3. Department of Clinical Microbiology, Rigshospitalet, University Hospital of Copenhagen, Denmark.
4. Guangxi Institute of Respiratory Diseases, First Affiliated Hospital of Guangxi Medical University, Nanning, Guangxi 530021, China. Email: chenyq163@tom.com.
Publication Type:Journal Article
MeSH: Animals; Biofilms; drug effects; Catheters, Indwelling; microbiology; Female; Levofloxacin; therapeutic use; Mice; Pseudomonas Infections; prevention & control; Pseudomonas aeruginosa; pathogenicity
From: Chinese Medical Journal 2014;127(1):54-58
CountryChina
Language:English
Abstract:
BACKGROUNDImplanted medical catheter-related infections are increasing, hence a need for developing catheter polymers bonded to antimicrobials. We evaluated preventive effects of levofloxacin-impregnated catheters in catheter-related Psuedomonas aeruginosa (strain PAO1) infection.
METHODSDrug release from levofloxacin-impregnated catheters was measured in vitro. Levofloxacin-impregnated catheters and polyvinyl chloride (PVC) catheters were immersed in 5 ml 50% Luria Bertani medium containing 10(8) CFU/ml Pseudomonas aeruginosa then incubated for 6, 12, 24 or 48 hours at 37°C when bacteria adhering to the catheters and bacteria in the growth culture medium were determined. Impregnated and PVC catheters were singly implanted subcutaneously in mice, 50 µl (10(7)CFU) of PAO1 was injected into catheters. After the first and fifth days challenge, bacterial counts on implanted catheters and in surrounding tissues were determined microbiologically. Bacterial colonization and biofilm formation on implanted catheters were assessed by scanning electron microscopy.
RESULTSDrug release from levofloxacin-impregnated catheters was rapid. Levofloxacin-impregnated catheters had significantly fewer bacteria compared to PVC in vitro. After first and fifth day of challenge, no or significantly fewer bacteria adhered to impregnated catheters or in surrounding tissues compared to PVC. Scanning electron microscopical images after first day displayed from none to significantly fewer bacteria adhering to impregnated implanted catheters, compared to bacteria and microcolonies adhering to PVC catheters. After the fifth day, no bacteria were found on impregnated catheters, compared to clusters surrounding mucus-like substance and coral-shaped biofilms with polymorphonuclear leukocyte on PVC catheters. After the first day of challenge, secretion occurred in all implanted catheters with surrounding tissues mildly hyperaemic and swollen. After the fifth day, minute secretions inside impregnated catheters and no inflammation in tissues, whereas purulent secretion inside PVC catheters and abscesses in surrounding tissues.
CONCLUSIONLevofloxacin-impregnated catheter is a promising new strategy for prevention of catheter-related Psuedomonas aeruginosa infection.