Mitochondrial apoptosis of lymphocyte is induced in type 2 diabetes.

Hui XU; Yanbo Chen; Yanxiang LI; Fangzhen Xia; Bing Han; Huixin Zhang; Hualing Zhai; Hui WU; Ying LI; Yingli LU

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Mitochondrial apoptosis of lymphocyte is induced in type 2 diabetes.

Author: Hui XU ¹ ; Yanbo CHEN ² ; Yanxiang LI ¹ ; Fangzhen XIA ¹ ; Bing HAN ¹ ; Huixin ZHANG ¹ ; Hualing ZHAI ¹ ; Hui WU ¹ ; Ying LI ¹ ; Yingli LU ³
Author Information

1. Endocrinology and Metabolism Research Institute and Department of Endocrinology and Metabolism, Shanghai Ninth People's Hospital Affiliated to Shanghai Jiaotong University School of Medicine, Shanghai 200011, China.
2. Department of Urology, Shanghai Ninth People's Hospital Affiliated to Shanghai Jiaotong University School of Medicine, Shanghai 200011, China.
3. Endocrinology and Metabolism Research Institute and Department of Endocrinology and Metabolism, Shanghai Ninth People's Hospital Affiliated to Shanghai Jiaotong University School of Medicine, Shanghai 200011, China. Email: luyingli2008@126.com.
Publication Type:Journal Article
MeSH: Aged; Aged, 80 and over; Apoptosis; physiology; Caspase 3; genetics; Caspase 9; genetics; Diabetes Mellitus, Type 2; metabolism; Female; Humans; Lymphocytes; cytology; metabolism; ultrastructure; Male; Microscopy, Electron, Transmission; Mitochondria; metabolism; Real-Time Polymerase Chain Reaction; bcl-2-Associated X Protein; genetics
From: Chinese Medical Journal 2014;127(2):213-217
CountryChina
Language:English
Abstract:
BACKGROUNDLymphocyte function and homeostasis is associated with immune defence to infection. Apoptosis of lymphocytes might be a considerably important component which has an impact on immunity to infections in people with hyperglycemia. The aim of this study was to explore the mitochondrial apoptosis pathway of lymphocyte in diabetic patients.
METHODSSixty patients with type 2 diabetes mellitus and fifty healthy volunteers were included in this study. Annexin V and propidiumiodide (PI) were joined in the isolated lymphocytes and the rate of lymphocyte apoptosis was calculated with flow cytometry. Observation of the lymphocytes was done using transmission electron microscopy; mitochondria had been extracted and then mitochondrial membrane potential (MMP) was detected to assess mitochondrial function; the mRNA level of Bcl-2, cytochrome c (Cyt-C), caspase-9 and caspase-3 were analyzed by real-time reverse transcription-polymerase chain reaction (RT-PCR).
RESULTSApoptosis rate of lymphocyte was significantly higher in diabetic group than that in normal control group (P < 0.05). Transmission electron microscopy showed lymphocyte shrinkage and breakage, chromatin condensation and less mitochondria; a fall in MMP levels was also evident; Bcl-2 concentration was reduced and the expressions of caspase-9, caspase-3 and Cyt-C were elevated (P < 0.05) in diabetic patients.
CONCLUSIONSThe rate of lymphocyte apoptosis was significantly higher in type 2 diabetic patients than that in normal population. Mitochondrial apoptosis pathway may play a very important role in decreasing function of lymphocyte in diabetes.