A rudimentary study of the acid fibroblast growth factor's plant expression vector construction and transformation tobacco.
- Author:
Shicui JIANG
1
;
Yi WANG
;
Xiaokun LI
;
Xiuwen HAN
;
Meiping ZHANG
Author Information
1. College of Life Sciences, Jilin Agriculture University, Changchun 130118, China.
- Publication Type:Journal Article
- MeSH:
Agrobacterium;
genetics;
Fibroblast Growth Factor 1;
biosynthesis;
genetics;
Genetic Vectors;
genetics;
Plants, Genetically Modified;
genetics;
metabolism;
Recombinant Proteins;
biosynthesis;
genetics;
Tobacco;
genetics;
metabolism
- From:
Journal of Biomedical Engineering
2010;27(1):126-131
- CountryChina
- Language:Chinese
-
Abstract:
Acid fibroblast growth factor (aFGF) has great potential in clinical application, but it is very expensive. In order to reduce the cost of production and to make full use of the merits integrated with plant bioreator, we have explored the aFGF in transgenic Tobacco expression. AFGF gene was inserted into plant expression vector pBI121; the acquired plants contained aFGF gene expression vector pBI121-TOAB-aF. Using Agrobacterium-mediated gene transformation of Tobacco and using transgenic Tobacco containing kanamycin and cephalosporin culture medium, we obtained kanamycin resistant transgenic Tobacco plants. PCR detection, RT-PCR detection and Western blot detection confirmed that foreign genes were successfully expressed in Tobacco. These data could serve as a theoretical foundation on which to use the plant bioreactor for production of aFGF.
