Cloning and expression of new genotype of CYP2C9 from Chinese Han in Escherichia coli.
- Author:
Pinghu ZHANG
1
;
Juan ZHANG
;
Yixiang GAO
;
Jing SHANG
;
Zhenzhou JIANG
;
Luyong ZHANG
Author Information
1. Jiangsu Center for New Drug Screening, China Pharmaceutical University, Nanjing 210009, China.
- Publication Type:Journal Article
- MeSH:
Aryl Hydrocarbon Hydroxylases;
biosynthesis;
genetics;
China;
ethnology;
Cloning, Molecular;
Cytochrome P-450 CYP2C9;
Escherichia coli;
genetics;
metabolism;
Genetic Vectors;
genetics;
Humans;
Point Mutation;
Polymorphism, Genetic;
Recombinant Proteins;
biosynthesis;
genetics
- From:
Journal of Biomedical Engineering
2010;27(2):365-368
- CountryChina
- Language:Chinese
-
Abstract:
In this study, we successfully expanded a full length gene encoding the monooxygenase eytochrome P450 2C9 gene from human liver of Chinese Han by RT-PCR. Our findings indicated that except G-->T mutation at the 190th nucleotide site, the other nucleotide sequences are completely consistent with CYP2C9 (NM017460) in GenBank. The SDS-PAGE and Western-Blot analysis showed that the CYP 2C9 gene was successfully expressed in the host cell E. coli BL21 (DE3). Our current study lays the foundation for the evaluation of pre-clinical drug metabolism and safety in the future.
