Purification and identification of some characteristics of lectin from stament of Lotus (Nelumbo nucifera) and flowers of Telosma cordata
- Author:
Khang Quoc Nguyen
- Publication Type:Journal Article
- Keywords:
Traditional medicine
- MeSH:
Lectins;
Lotus;
Plants, Medicinal;
Medicine, Traditional
- From:Pharmaceutical Journal
2001;298(2):15-18
- CountryViet Nam
- Language:Vietnamese
-
Abstract:
The Lectin from Nulumbo nucifera and Telosma cordata have been extracted and purified by following process: Extraction with suitable medium precipitation with Ammoniumsunphate or Ethanol - ion exchange chromatography on CM-Cellulose of DEAE-Cellulose. Preparations having high purity showed homogeneous proteins in SDS-polyacrylamide gel-electrophoresis. pH-Optimum of the lectins from Nelumbo nucifera is pH 8.3, but form Telosma cordata is pH 5.5. The potency order of agglutination with monosaccharides was as Fructose, NAc-Glucosamine, Arabinose and NAc-acid Neuraminic for Nelumbo nucifera, but the lectin from Telosma cordata was inhibited only by Fructose, Saccharose NAc acid neuraminic. The lectins from Nelumbo nucifera and Telosma cordata were inhibited by many proteins from original Animals
