Application of trichloroacetic acid-acetone precipitation method for protein extraction in bone tissue.
- Author:
Cui YONG-FENG
1
;
Yang ZHEN
;
Zhu BAO-HUA
;
Xiao LU-WEI
;
Tong PEI-JIAN
Author Information
- Publication Type:Journal Article
- MeSH: Acetone; Animals; Bone and Bones; chemistry; Chemical Precipitation; Male; Proteins; isolation & purification; Proteomics; methods; Rats; Rats, Wistar; Trichloroacetic Acid
- From: Acta Academiae Medicinae Sinicae 2011;33(2):210-213
- CountryChina
- Language:English
-
Abstract:
OBJECTIVETo explore the feasibility and efficiency of extracting protein from bone tissue using trichloroacetic acid (TCA)-acetone precipitation method.
METHODSHydrochloric acid (HCL) decalcification method and TCA-acetone precipitation method were separately used for bone protein extraction. The efficiencies of these two methods were compared.
RESULTSTCA-acetone precipitation method had significantly higher extraction efficiency. Compared with HCL decalcification method, it had less pollution from bone marrow hematopoietic tissue. Protein band distribution was similar between these two methods.
CONCLUSIONTCA-acetone precipitation method is useful for bone proteomics research.
